An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations
نویسندگان
چکیده
منابع مشابه
Discovery of protein‐protein interactions by affinity purification and mass spectrometry (AP‐MS)
Cells/tissues are then lysed and the Ab is added. Recovery of the Ab‐ t‐X complex is then done mostly using a capture resin made of ProteinA‐coated beads which bind the Fc portion of the Ab. However other systems exist which rely on other types of affinity interactions, i.e. biotinylation (strept)avidin systems or (His)6 Ni2+ columns etc. It is important to note that, several interactions (o...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2018
ISSN: 2041-1723
DOI: 10.1038/s41467-018-03523-2